Objectives

To evaluate the antibacterial and mineralization properties of a dental adhesive containing Ag/polydopamine-modified HA (HA, hydroxyapatite) fillers.

Methods

First, an HA-polydopamine-Ag-polydopamine (HA-PDA-Ag-PDA) filler was prepared and characterized using SEM, TEM, XPS, XRD and FTIR. Then, the HA-PDA-Ag-PDA filler was mixed into an adhesive at different mass fractions (0 wt%, 0.5 wt%, 1 wt%, 2 wt%) to prepare a functional adhesive. Antibacterial and mineralization tests were carried out, and the cytotoxicity of the functional adhesive against L929 fibroblasts was also examined.

Results

The SEM, TEM, XPS, XRD and FTIR characterizations confirmed the successful preparation of the HA-PDA-Ag filler. The 1 wt% and 2 wt% functional adhesives showed the strongest bacterial inhibition effect among all the samples (p < 0.05). Obvious apatite crystals were observed in the SEM micrograph of the surface of the functional adhesive sample after immersion in artificial saliva for predetermined times (1 d, 7 d, 14 d and 28 d). There was no significant difference between the experimental group and the control group in terms of cell proliferation activity (p > 0.05).

Conclusions

The 1 wt% and 2 wt% functional adhesives demonstrated good antibacterial and mineralization properties, as well as good biocompatibility.

Clinical significance

Functional adhesives containing Ag/polydopamine-modified HA fillers with antibacterial and mineralization capabilities might have excellent potential to enhance the stability and durability of hybrid layers and prolong the service life of dental restorations. Our study on bifunctional adhesives has paved the way for future clinical applications to increase restoration longevity.

中文翻译：

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一种新型牙科粘合剂，含有具有抗菌和矿化特性的 Ag/聚多巴胺改性 HA 填料

目标

评估含有银/聚多巴胺改性的 HA（HA，羟基磷灰石）填料的牙科粘合剂的抗菌和矿化特性。

方法

首先，制备了 HA-聚多巴胺-Ag-聚多巴胺 (HA-PDA-Ag-PDA) 填料，并使用 SEM、TEM、XPS、XRD 和 FTIR 对其进行了表征。然后，将HA-PDA-Ag-PDA填料以不同的质量分数（0wt%、0.5wt%、1wt%、2wt%）混合到粘合剂中以制备功能性粘合剂。进行了抗菌和矿化测试，并检查了功能性粘合剂对 L929 成纤维细胞的细胞毒性。

结果

SEM、TEM、XPS、XRD 和 FTIR 表征证实了 HA-PDA-Ag 填料的成功制备。1 wt% 和 2 wt% 的功能性粘合剂在所有样品中表现出最强的细菌抑制作用（p < 0.05）。在人工唾液中浸泡预定时间（1 d、7 d、14 d和28 d）后，功能粘合剂样品表面的SEM显微照片中观察到明显的磷灰石晶体。实验组与对照组细胞增殖活性无显着差异（p > 0.05）。

结论

1 wt% 和 2 wt% 的功能性粘合剂表现出良好的抗菌和矿化性能，以及良好的生物相容性。

临床意义

含有具有抗菌和矿化能力的 Ag/聚多巴胺改性 HA 填料的功能性粘合剂可能具有增强混合层的稳定性和耐久性并延长牙科修复体使用寿命的巨大潜力。我们对双功能粘合剂的研究为未来的临床应用铺平了道路，以延长修复体的寿命。