Senescent cells are characterized by an arrest in proliferation. In addition to replicative senescence resulting from telomere exhaustion, sub-lethal genotoxic stress resulting from DNA damage, oncogene activation, mitochondrial dysfunction or reactive metabolites also elicits a senescence phenotype. Senescence is a controlled programme affecting a wide variety of biological processes with some core hallmarks of senescence as well as tissue specific changes. This study presents an integrative multi-omic analysis of proteomic and RNA-seq from proliferating and senescent osteosarcoma cells. This study demonstrates senescence induction in a widely used cell line which can be used as a model system for characterising cancer cell responses to sub-lethal doses of chemotherapeutic agents, and makes available both RNA-seq and proteomic data from proliferating and senescent cells in open access repositories to aid reuse by the community.

中文翻译：

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癌细胞系药物诱导衰老的综合 RNA 蛋白质组学景观

衰老细胞的特征在于增殖停滞。除了端粒耗竭导致的复制性衰老外，DNA 损伤、致癌基因激活、线粒体功能障碍或反应性代谢物导致的亚致死基因毒性应激也会引发衰老表型。衰老是一个受控程序，影响着多种生物过程，具有衰老的一些核心特征以及组织特异性变化。本研究对增殖和衰老的骨肉瘤细胞的蛋白质组学和 RNA-seq 进行了综合多组学分析。这项研究证明了广泛使用的细胞系中的衰老诱导，该细胞系可用作表征癌细胞对亚致死剂量化疗药物反应的模型系统，