Cardiovascular diseases are the leading cause of death globally. Vascular implants, such as stents, are required to treat arterial stenosis or dilatation. The development of innovative stent materials and coatings, as well as novel preclinical testing strategies, is needed to improve the bio- and hemocompatibility of current stents. In this study, a blood vessel-like polydimethylsiloxane (PDMS) model was established to analyze the interaction of an endothelium with vascular implants, as well as blood-derived cells, in vitro. Using footprint-free human induced pluripotent stem cells (hiPSCs) and subsequent differentiation, functional endothelial cells (ECs) expressing specific markers were generated and used to endothelialize an artificial PDMS lumen. The established model was used to demonstrate the interaction of the created endothelium with blood-derived immune cells, which also allowed for real-time imaging. In addition, a stent was inserted into the endothelialized lumen to analyze the surface endothelialization of stents. In the future, this blood vessel-like model could serve as an in vitro platform to test the influence of vascular implants and coatings on endothelialization and to analyze the interaction of the endothelium with blood cell components.

中文翻译：

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使用无足迹 hiPSC 产生的自体内皮细胞开发体外血管模型，以分析内皮与血细胞成分和血管植入物的相互作用

心血管疾病是全球死亡的主要原因。血管植入物，如支架，需要治疗动脉狭窄或扩张。需要开发创新的支架材料和涂层，以及新的临床前测试策略，以提高当前支架的生物和血液相容性。在这项研究中，建立了一种血管样聚二甲基硅氧烷 (PDMS) 模型，以在体外分析内皮与血管植入物以及血源性细胞的相互作用。使用无足迹的人类诱导多能干细胞 (hiPSC) 和随后的分化，生成表达特定标记的功能性内皮细胞 (EC) 并用于内皮化人工 PDMS 腔。建立的模型用于演示创建的内皮细胞与血液来源的免疫细胞的相互作用，这也允许进行实时成像。此外，将支架插入内皮化管腔以分析支架的表面内皮化。将来，这种类似血管的模型可以作为体外平台来测试血管植入物和涂层对内皮化的影响，并分析内皮与血细胞成分的相互作用。