Similar to hypochlorous acid (HClO), hypobromous acid (HBrO) is one of the most notable reactive oxygen species (ROS). Overexpression of HBrO is linked to various diseases causing organ and tissue loss. Due to HBrO's role in the oxidation of micropollutants, real-time monitoring of HBrO in water-based systems is essential. Tetraphenylethylene (TPE)-based organic aggregation-induced emission luminophores (AIEgens) are an emerging category of fluorescent probe materials that have attracted considerable attentions. However, AIE probes are rarely applied to detect HBrO. Developing faster, more precise, and more sensitive AIE probes is thus crucial for detecting biological and environmental HBrO. A small molecule fluorescent probe 4-(1,2,2-triphenylvinyl)benzamidoxime () was synthesized for the sensitive and selective detection of hypobromous acid (HBrO) based on aggregation-induced emission (AIE). The amidoxime unit of would undergo an oxidation reaction with HBrO, leading to a structure differentiation between the probe and the product, and therefore the turn-on fluorescence by the AIE effect. The probe could recognize hypobromous acid rapidly (less than 3 s) in high aqueous phase (99 % water) with a turn-on fluorescence response. It was determined that the limit of detection for HBrO was 5.47 nM. Moreover, demonstrates potential as a test strip for the detection of HBrO. was also successfully used for the monitoring of HBrO in water samples and for the detection of endogenous/exogenous HBrO in living cells and zebrafish. A special AIE fluorescence turn-on probe based on tetraphenylethylene was designed for detecting HBrO in the environmental and biological systems. This probe has an extremely low detection limit of 5.47 nM and is able to detect HBrO in 99 % aqueous phase in less than 3 s.

中文翻译：

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基于四苯乙烯的荧光AIE探针快速检测次溴酸及其应用

与次氯酸 (HClO) 类似，次溴酸 (HBrO) 是最著名的活性氧 (ROS) 之一。 HBrO 的过度表达与导致器官和组织损失的多种疾病有关。由于 HBrO 在微污染物氧化中的作用，实时监测水基系统中的 HBrO 至关重要。基于四苯乙烯（TPE）的有机聚集诱导发射发光体（AIEgens）是一类新兴的荧光探针材料，引起了人们的广泛关注。然而，AIE 探针很少用于检测 HBrO。因此，开发更快、更精确、更灵敏的 AIE 探针对于检测生物和环境 HBrO 至关重要。合成了一种小分子荧光探针 4-(1,2,2-三苯基乙烯基)苯甲肟 ()，用于基于聚集诱导发射 (AIE) 的次溴酸 (HBrO) 的灵敏和选择性检测。的偕胺肟单元会与HBrO发生氧化反应，导致探针和产物之间的结构差异，从而通过AIE效应开启荧光。该探针可以在高水相（99% 水）中快速（小于 3 秒）识别次溴酸，并发出开启荧光响应。确定 HBrO 的检测限为 5.47 nM。此外，还展示了作为检测 HBrO 的测试条的潜力。还成功用于水样中 HBrO 的监测以及活细胞和斑马鱼中内源/外源 HBrO 的检测。设计了一种基于四苯乙烯的特殊 AIE 荧光开启探针，用于检测环境和生物系统中的 HBrO。该探针的检测限极低，为 5.47 nM，能够在 3 秒内检测 99% 水相中的 HBrO。