While levodopa (L‐Dopa) is the primary treatment for alleviating Parkinson's disease (PD), its efficacy is hindered by challenges such as a short half‐life and inconsistent plasma levels. As PD progresses, the rising need for increased and more frequent L‐Dopa doses coupled with symptom fluctuations and dyskinesias underscores the urgency for improved comprehension of the interplay between L‐Dopa levels and PD motor symptoms. Addressing this critical need, we present a decentralized testing method using a disposable biosensor strip and a universal slope (U‐slope) calibration‐free approach. This enables reliable, rapid, simple, and cost‐effective decentralized L‐Dopa measurements from capillary blood. A pilot study with PD persons demonstrates the ability to monitor real‐time L‐Dopa pharmacokinetics from fingerstick blood after oral L‐Dopa‐Carbidopa (C‐Dopa) tablet administration. Correlating capillary blood L‐Dopa levels with PD motor scores revealed a well‐defined inverse correlation with temporal motor fluctuations. We compared the resulting dynamic capillary blood L‐Dopa levels with plasma L‐Dopa levels using the traditional but clinically impractical high‐performance liquid chromatography technique. By providing timely feedback on a proper L‐Dopa dosing regimen in a decentralized and rapid fashion, this new biosensing platform will facilitate tailored optimal L‐Dopa dosing, towards improving symptom management and enhancing health‐related quality of life.

中文翻译：

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用于快速现场评估帕金森病左旋多巴药代动力学和运动表现的生物传感器带

虽然左旋多巴（L-Dopa）是缓解帕金森病（PD）的主要治疗方法，但其疗效受到半衰期短和血浆水平不一致等挑战的阻碍。随着帕金森病的进展，对左旋多巴剂量的增加和更频繁的需求不断增加，加上症状波动和运动障碍，强调了提高对左旋多巴水平和帕金森病运动症状之间相互作用的理解的紧迫性。为了满足这一关键需求，我们提出了一种使用一次性生物传感器带和通用斜率（U 斜率）免校准方法的分散测试方法。这样可以实现可靠、快速、简单且经济高效的毛细血管血分散左旋多巴测量。一项针对 PD 患者的初步研究表明，口服左旋多巴卡比多巴 (C-Dopa) 片剂后，能够通过指尖采血监测实时左旋多巴药代动力学。将毛细血管血左旋多巴水平与 PD 运动评分相关联，发现与时间运动波动存在明确的负相关。我们使用传统但临床上不实用的高效液相色谱技术，将所得的动态毛细血管血左旋多巴水平与血浆左旋多巴水平进行了比较。通过以分散和快速的方式及时提供适当的左旋多巴给药方案的反馈，这个新的生物传感平台将促进量身定制的最佳左旋多巴剂量，从而改善症状管理并提高与健康相关的生活质量。