The diagnosis of malignant melanoma, often an inconspicuous but highly aggressive tumor, is most commonly done by histological examination, while additional diagnostic methods on the level of elements and molecules are constantly being developed. Several studies confirmed differences in the chemical composition of healthy and tumor tissue. Our study presents the potential of the LIBS (Laser-Induced-Breakdown Spectroscopy) technique as a diagnostic tool in malignant melanoma (MM) based on the quantitative changes in elemental composition in cancerous tissue. Our patient group included 17 samples of various types of malignant melanoma and one sample of healthy skin tissue as a control. To achieve a clear perception of results, we have selected two biogenic elements (calcium and magnesium), which showed a dissimilar distribution in cancerous tissue from its healthy surroundings. Moreover, we observed indications of different concentrations of these elements in different subtypes of malignant melanoma, a hypothesis that requires confirmation in a more extensive sample set. The information provided by the LIBS Imaging method could potentially be helpful not only in the diagnostics of tumor tissue but also be beneficial in broadening the knowledge about the tumor itself.

中文翻译：

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使用激光诱导击穿光谱对人类恶性黑色素瘤内的元素分布进行成像


恶性黑色素瘤通常是一种不起眼但具有高度侵袭性的肿瘤，其诊断最常通过组织学检查来完成，同时在元素和分子水平上的其他诊断方法也在不断开发。几项研究证实了健康组织和肿瘤组织的化学成分存在差异。我们的研究基于癌组织中元素成分的定量变化，展示了 LIBS（激光诱导击穿光谱）技术作为恶性黑色素瘤 (MM) 诊断工具的潜力。我们的患者组包括 17 份各种类型的恶性黑色素瘤样本和一份健康皮肤组织样本作为对照。为了获得清晰的结果感知，我们选择了两种生物元素（钙和镁），它们在癌组织中的分布与其健康环境不同。此外，我们观察到这些元素在不同亚型的恶性黑色素瘤中存在不同的浓度，这一假设需要在更广泛的样本集中得到证实。 LIBS 成像方法提供的信息不仅可能有助于肿瘤组织的诊断，而且有利于拓宽有关肿瘤本身的知识。