The spontaneous solid-state stacking process (SSSP) of is an environmentally friendly and cost-effective process for enriching and assembling environmental microorganisms to guarantee the subsequent fermentation efficiency. In this study, how SSSP create spatial heterogeneity of stacking piles were found through spatiotemporal sampling. The degree of difficulty in oxygen exchange categorizes the stacking pile into depleted (≤4%), transitional (4 %–17 %), and enriched (≥17 %) oxygen-defined layers. This results in variation in succession rates (V > V > V), which accelerates spatial heterogeneity during SSSP. As a dominant species (65 %–99 %) in depleted and transitional layers, can rapidly reduce oxygen disturbance by upregulating and , that sustains spatial heterogeneity. The findings demonstrated the value of oxygen control in shaping spatial heterogeneity during SSSP processes, which can create specific functional microbiome. Adding spatial heterogeneity management will help achieve more precise control of such solid-state fermentation systems.

中文翻译：

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氧气影响白酒堆积过程中的空间异质性和微生物演替动态


自发固态堆积工艺（SSSP）是一种环境友好、经济高效的工艺，用于富集和组装环境微生物，保证后续发酵效率。本研究通过时空采样发现了SSSP如何造成堆垛空间异质性。氧交换的困难程度将堆垛分为贫氧层（≤4%）、过渡层（4%–17%）和富氧层（≥17%）。这会导致演替率的变化 (V > V > V)，从而加速 SSSP 期间的空间异质性。作为贫化层和过渡层中的优势物种 (65%–99%)，可以通过上调 和 来快速减少氧干扰，从而维持空间异质性。研究结果证明了氧控制在 SSSP 过程中塑造空间异质性方面的价值，这可以创建特定的功能微生物组。增加空间异质性管理将有助于实现对此类固态发酵系统的更精确控制。