Genome editing is a crucial technology for obtaining desired phenotypes in a variety of species, ranging from microbes to plants, animals, and humans. With the advent of CRISPR-Cas technology, it has become possible to edit the intended sequence by modifying the target recognition sequence in guide RNA (gRNA). By expressing multiple gRNAs simultaneously, it is possible to edit multiple targets at the same time, allowing for the simultaneous introduction of various functions into the cell. This can significantly reduce the time and cost of obtaining engineered microbial strains for specific traits. In this review, we investigate the resolution of multiplex genome editing and its application in engineering microorganisms, including bacteria and yeast. Furthermore, we examine how recent advancements in artificial intelligence technology could assist in microbial genome editing and engineering. Based on these insights, we present our perspectives on the future evolution and potential impact of multiplex genome editing technologies in the agriculture and food industry.

中文翻译：

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多重 CRISPR-Cas 基因组编辑：下一代微生物菌株工程


基因组编辑是在从微生物到植物、动物和人类的各种物种中获得所需表型的关键技术。随着CRISPR-Cas技术的出现，通过修改引导RNA（gRNA）中的目标识别序列来编辑预期序列已成为可能。通过同时表达多个 gRNA，可以同时编辑多个靶标，从而将各种功能同时引入细胞中。这可以显着减少获得具有特定性状的工程微生物菌株的时间和成本。在这篇综述中，我们研究了多重基因组编辑的分辨率及其在工程微生物（包括细菌和酵母）中的应用。此外，我们研究了人工智能技术的最新进展如何协助微生物基因组编辑和工程。基于这些见解，我们提出了对农业和食品行业多重基因组编辑技术的未来发展和潜在影响的看法。